| feature | ||||||
|---|---|---|---|---|---|---|
| General summary | H9_2_4 | H9_3_4 | H9_4_4 | HEYA8_1_4 | HEYA8_2_4 | HEYA8_3_3 |
| Mean read length | 1,025.7 | 1,031.0 | 1,041.8 | 1,006.4 | 986.7 | 1,005.4 |
| Mean read quality | 10.1 | 10.1 | 10.2 | 10.1 | 10.1 | 10.1 |
| Median read length | 805.0 | 812.0 | 819.0 | 793.0 | 775.0 | 787.0 |
| Median read quality | 11.3 | 11.4 | 11.4 | 11.3 | 11.4 | 11.3 |
| Number of reads | 25,990,415.0 | 26,661,355.0 | 23,772,988.0 | 27,541,966.0 | 27,155,084.0 | 24,418,058.0 |
| Read length N50 | 1,247.0 | 1,231.0 | 1,253.0 | 1,143.0 | 1,114.0 | 1,132.0 |
| STDEV read length | 784.7 | 760.7 | 783.6 | 709.8 | 697.9 | 731.9 |
| Total bases | 26,658,830,735.0 | 27,487,267,637.0 | 24,766,107,246.0 | 27,717,191,395.0 | 26,793,370,893.0 | 24,549,191,362.0 |
| Number, percentage and megabases of reads above quality cutoffs | ||||||
| >Q10 | 18333209 (70.5%) 19944.0Mb | 18990705 (71.2%) 20749.8Mb | 17013013 (71.6%) 18803.1Mb | 19453279 (70.6%) 20583.7Mb | 19423599 (71.5%) 20086.4Mb | 17356578 (71.1%) 18357.6Mb |
| >Q15 | 599798 (2.3%) 996.6Mb | 588164 (2.2%) 988.0Mb | 540785 (2.3%) 910.4Mb | 570784 (2.1%) 918.9Mb | 593823 (2.2%) 919.6Mb | 485365 (2.0%) 791.3Mb |
| >Q20 | 22 (0.0%) 0.0Mb | 32 (0.0%) 0.0Mb | 32 (0.0%) 0.0Mb | 16 (0.0%) 0.0Mb | 29 (0.0%) 0.0Mb | 6 (0.0%) 0.0Mb |
| >Q25 | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb |
| >Q30 | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb | 0 (0.0%) 0.0Mb |
| Top 5 highest mean basecall quality scores and their read lengths | ||||||
| 1 | 22.9 (243) | 22.7 (288) | 22.5 (351) | 22.2 (589) | 22.4 (257) | 20.6 (503) |
| 2 | 21.8 (284) | 21.8 (471) | 22.3 (303) | 21.8 (595) | 21.8 (156) | 20.2 (236) |
| 3 | 21.4 (146) | 21.2 (434) | 22.2 (270) | 21.4 (513) | 21.8 (393) | 20.1 (380) |
| 4 | 21.2 (602) | 21.2 (343) | 21.6 (233) | 21.4 (479) | 21.2 (211) | 20.1 (205) |
| 5 | 21.2 (243) | 21.2 (380) | 21.5 (294) | 21.3 (167) | 20.9 (209) | 20.1 (528) |
| Top 5 longest reads and their mean basecall quality score | ||||||
| 1 | 360421 (6.8) | 420040 (3.7) | 479573 (3.8) | 511241 (6.2) | 527046 (7.9) | 615873 (11.3) |
| 2 | 328832 (5.2) | 296468 (3.1) | 406113 (3.6) | 418712 (8.5) | 284538 (5.9) | 543465 (4.0) |
| 3 | 234353 (4.2) | 294030 (3.8) | 294690 (4.0) | 293430 (6.3) | 282840 (5.4) | 411550 (3.9) |
| 4 | 230999 (5.0) | 224502 (5.4) | 269667 (5.3) | 218968 (5.9) | 275873 (7.5) | 267342 (3.3) |
| 5 | 216349 (3.3) | 211121 (5.0) | 262977 (5.3) | 185502 (5.1) | 251671 (6.6) | 223831 (6.5) |